Reporter

Part:BBa_K352011:Design

Designed by: Ş.Serap Memik   Group: iGEM10_METU_Turkey   (2010-10-16)

pCooM Promoter coupled with RBS+RFP+Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Device created from a composite of parts.



The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks.


References


- Aono S, Ohkubo K, Matsuo T, and Nakajima T (1998). Redox-controlled ligand exchange of the Heme in the CO-sensing transcriptional activator CooA. The Journal of Biologycal Chemistry 1998; 273(40): 25757-64.


- Aono S (2003). Biochemical and Biophysical Properties of the CO-Sensing Transcriptional Activator CooA. Accounts of Chemical Research 2003; 36(11): 825-831.